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Scanning of Hybridised Slide(s)

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Once hybridisation has been completed, microarray slide(s) are scanned with an Affymetrix 418 scanner which generates two colour images (Cy3 & Cy5). These are saved as .tif files. The scanned images are then analysed with image quantification software programs e.g. ImaGene (Biodiscovery, Santa Monica, CA). This generates spot (gene) identification and quantitation files (.txt files). These can then be loaded into further downstream analysis software.

For more information on the analysis side of microarrays, visit the Bioinformatics Section.

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